The determination of the sex of Simarouba seedling prior to the flowering stage would avoid the need for removing undesired sex male plants from the field. There is no method available to distinguish male, female, and hermaphrodite plants in pre-flowering stage in Simarouba. Subsequently, quality and quantity were checked by running the dissolved DNA in 0. Leaf samples were collected from male, female and hermaphrodite plants after complete observation of flower types and these were used for DNA extraction. Materials and methods 2. Random amplified polymorphic DNA primers were tested on dioeceious and hermaphrodite plant Simarouba glauca. Simarouba belongs to Simaroubaceae family, is indigenous to the Amazon rainforest and other tropical areas in Mexico, Cuba, Haiti, Jamaica, and Central America. The agarose gels were stained with 0.
Primer OPW produced amplicon specific to male and hermaphrodite plants and was absent in female plants. Published by Elsevier B. Simarouba glauca is a tree born oilseed crop. Vegetable oil is a promising alternative because it has several advantages, viz it is renewable, environment friendly and produced easily in rural area, where there is acute need for modern forms of energy. The OPS primer could also be used to discriminate male from female and hermaphrodite plants. Thus, an increase in the number of fruit-bearing plants per hectare of land would directly increase the total yield in the field making its cultivation more profitable. The extraction steps were repeated using the same phenol: The development of molecular strategies for early sex detection of dioecious plants has been a priority in breeding programs for their greater economic potentials. Molecular marker based technology has been proved a reliable strategy for detection of sex-associated markers in dioecious and bisexual plants. The objective of this study is to identify molecular markers linked to sex determination of the plant Simarouba glauca which assists in crop improvement program. Simarouba species has the ability to substitute the requirement of low cost feed stock with the potential for high oil seed production and added benefit of an ability to grow on marginal land. Subsequently, quality and quantity were checked by running the dissolved DNA in 0. Materials and methods 2. The determination of the sex of Simarouba seedling prior to the flowering stage would avoid the need for removing undesired sex male plants from the field. RAPD marker analysis Eighty five base primers Operon Technologies, Alameda, USA were used for polymerase chain reaction PCR for screening of known sex to ascertain their potential of clear amplification in polymorphism and also the reproducibility. Random amplified polymorphic DNA primers were tested on dioeceious and hermaphrodite plant Simarouba glauca. There is no method available to distinguish male, female, and hermaphrodite plants in pre-flowering stage in Simarouba. RAPD markers have shown their reliability for determining sex in Pistacia vera  , Atriplex garrettii  , Trichosanthes diocia  , Salix viminalis  , Piper longum  , Borassus flabellifer  , Simmondsia chinensis  , Carica papaya, and Cycas circinalis  , Commiphora wightii . This property supports the suitability of Simarouba species for the sustainable biodiesel industry . The aim of present study is to indentify RAPD markers associated with sex determination in Simarouba. Female and hermaphrodite specific primer OPS amplified an amplicon in female and hermaphrodite and was absent in male plant. In addition, SCAR markers originating from RAPD markers were also developed for distinguishing the sex specificity in many plant species  ,  ,  ,  ,  , . The reactions were performed in a Master Cycler Gradient Eppendorf version 2. The agarose gels were stained with 0. Simarouba is commonly known as paradise tree, dysentery bark.
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